He has moved to a one-point lead over Democrat Ralph Northam after trailing the current lieutenant governor by five points in Monmouth's September poll.
It will be held at the Richmond Convention Center, according to an email sent to Northam supporters who applied for tickets to the rally.
600 likely voters took part in the poll.More news: NAFTA Trade Ministers to Square Off Over Hard-Line US Demands
In the Quinnipiac poll, Northam has strong support from independent voters with 53 percent of the support, compared to 39 percent for Gillespie.
"Virginians from across the Commonwealth support Dr. Northam because they know he will bring economic opportunity for all Virginians, no matter who they are or where they live", said campaign manager Brad Komar. Libertarian Cliff Hyra received 2 percent of the votes in that poll.
Almost half of the likely voters in Virginia (45%) say they see their vote for governor as a way to send a message to Trump and the Republicans who control Congress.More news: DOJ: 4 Cities May Have Sanctuary City Violations
In northern Virginia, however, more than half (54 percent) say Mr. Trump is a factor in their vote.
Obama plans to campaign for Northam in the state capital of Richmond Thursday night.
This poll comes just a day after a Wason Center poll showed Northam with a 4 point lead, which falls within that poll's margin of error. Trump lost Virginia to Hillary Clinton and Republicans in the Old Dominion have embraced the president at varying levels. Democrats are hoping anti-Trump sentiment will allow them to sweep all three statewide races and make serious gains in the Republican-controlled state House. Former President George W. Bush made stops at Gillespie fundraisers on Monday.More news: Trump on Iran nuke accord: It's the 'worst deal. We got nothing'